HAMTRAMCK, Mich. -- The last man to see a missing 2-year-old Hamtramck girl has taken a second lie-detector test.

Tangena Hussain has been missing since Oct. 2 when she disappeared while in Jamrul Hussain's care.

Hussain is not related to the child, but he is her mother’s boyfriend.

He already failed one polygraph test, and his lawyer agreed to have Hussain take a second lie-detector test.

On Friday, Local 4 learned Hussain was so emotional and upset during the polygraph, there are no usable results.

Last week, the FBI and police searched a third home in hopes of finding clues about Tangena's disappearance.

Detectives also executed a search warrant at Hussain's Hamtramck home.

On Friday, Local 4 learned blood evidence collected at the home does not match Tangena’s DNA.

Hussain told police he stopped at a Marathon gas station in Detroit at about 9 p.m. and left the girl strapped in the car while he went into the store.

He said when he returned, the girl was gone.

Hussain did not immediately report the girl missing and drove to pick up the child's mother, Nilufa Begum, who works at the Northland Mall.

Begum said when she saw her daughter’s sandals in the back of the car, she began to question Hussain.

"I was asking, 'Where's my daughter; tell me where's my daughter?' and he couldn't even say anything," said Begum.

Begum said Hussain took her back and recounted the incident to her.

"I said, 'What do you mean she's not inside; why didn’t you call the cops?'" said Begum. "He said, 'I was scared, and I wanted to get you first.'"

Surveillance tapes from the gas station were handed over to authorities, but investigators said a technical glitch prevented Hussain’s car and the child from being seen. Investigators said Hussain was filmed entering the store.

Investigators said without seeing the girl on tape, they cannot confirm that she was even taken from the gas station.

John Broad of Crime Stoppers said the organization received nearly a dozen confidential tips over the weekend about Tangena. "I'm sure there are more tips on the way," Broad said.

"This is too important. This is a little girl that needs our help," said Broad. "We've gotta find her."

Boyfriend Of Missing Girl's Mother Arraigned On Rape Charges

While authorities search for the missing girl, Hussain is engaged in a separate legal battle in an unrelated assault case.

The 24-year-old was arrested by Hamtramck police and charged with kidnapping and sexually assaulting a 15-year-old girl who was allegedly kept in a friend's basement.

Couple Arraigned On Kidnapping Charges In Connection With Missing Child Case